Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Long non-coding RNA KCNQ1 opposite transcript 1 (KCNQ1OT1) regulates cell proliferation and invasion via targeting miR-124-3p in gastric carcinoma

Xinying Ye¹, Hongfu Liu¹, Zhen Zeng²

¹Department of General Surgery, The First Affiliated Hospital of Gannan Medical University, PR China; ²CT Room, The First Affiliated Hospital of Gannan Medical University, PR China.

For correspondence:- Zhen Zeng Email: er56623@126.com Tel:+8618726520999

Accepted: 20 September 2020 Published: 31 January 2021

Citation: Ye X, Liu H, Zeng Z. Long non-coding RNA KCNQ1 opposite transcript 1 (KCNQ1OT1) regulates cell proliferation and invasion via targeting miR-124-3p in gastric carcinoma. Trop J Pharm Res 2021; 20(1):1-9 doi: 10.4314/tjpr.v20i1.1

© 2021 The authors.
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Abstract

Purpose: Long non-coding RNA (lncRNA) KCNQ1OT1 (KCNQ1 opposite strand/antisense transcript 1) has been associated with many kinds of cancers. However, its role hasn’t been researched much in gastric cancer. The present study investigated the role and mechanism of KCNQ1OT1 in gastric cancer.

Methods: Quantitative-PCR assays was used to measure KCNQ1OT1 and miR-124-3p expression in gastric cancer tissues and cells. Transfection assays were conducted to evealuate the regulation of KCNQ1OT1 or miR-124-3p expression in vitro. CCK-8 assays examined cell proliferation and Transwell invasion assays checked cell invasion abilities due to the changes of the key genes. On the other hand, luciferase reporter assays assisted in verifying the binding between KCNQ1OT1 and miR-124-3p. To explore the mechanism of KCNQ1OT1 in gastric cancer, we checked the independent influence of .KCNQ1OT1 or miR-124-3p on cellular functions and also examined the interplay between the two genes in gastric cancer cell lines.

Results: The findings showed that KCNQ1OT1 had high expression while miR-124-3p had low expression in gastric cancer tissues and cells in comparison with normal ones. KCNQ1OT1 was found to bind and target miR-124-3p. KCNQ1OT1 was shown to promote cell proliferation and invasion while miR-124-3p inhibit. Furthermore, the inhibition of miR-124-3p could partially reverse cell function changes induced by the knockdown of KCNQ1OT1 including cell viability, cell invasion and EMT process.

Conclusion: KCNQ1OT1 was a target of miR-124-3p and reduction in its expression led to an increase of miR-124-3p and inhibited the proliferation, invasion and EMT in Gastric Cancer. These results provided evidence that KCNQ1OT1 promoted the growth and invasion of the cells via targeting miR-124-3p in GC.

Keywords: KCNQ1OT1, miR-124-3p, gastric carcinoma, proliferation, invasion